Language
English
Publication Date
8-26-2025
Journal
Cell Reports
DOI
10.1016/j.celrep.2025.116070
PMID
40742812
PMCID
PMC12478994
PubMedCentral® Posted Date
9-30-2025
PubMedCentral® Full Text Version
Author MSS
Abstract
Injury causes differentiated cells to undergo massive reprogramming to become proliferative and repair tissue via paligenosis. Gastric chief cells use paligenosis to reprogram into progenitor-like spasmolytic-polypeptide-expressing metaplasia (SPEM) cells. Stage 1 of paligenosis is the downscaling of mature cell architecture via a process involving lysosomes. Here, we notice that sulfated glycoproteins are not only digested during paligenosis but also excreted into the gland. Various genetic and pharmacological approaches show that endoplasmic reticulum membranes and secretory granule cargo are also excreted and that the process proceeds in parallel with but is mechanistically independent of autophagy. Three-dimensional light and electron microscopy demonstrated that excretion occurs via unique, complex, multi-chambered invaginations of the apical plasma membrane. As this lysosome-independent cell cleansing process does not seem to have been priorly described, we termed it "cathartocytosis." Cathartocytosis allows a cell to rapidly eject excess material without waiting for autophagic and lysosomal digestion, providing for efficient cellular downscaling.
Keywords
Cell Differentiation, Animals, Cellular Reprogramming, Lysosomes, Autophagy, Humans, Mice, Endoplasmic Reticulum, Cell Membrane
Published Open-Access
yes
Recommended Citation
Brown, Jeffrey W; Lin, Xiaobo; Nicolazzi, Gabriel Anthony; et al., "Cathartocytosis: Jettisoning of Cellular Material During Reprogramming of Differentiated Cells" (2025). Faculty and Staff Publications. 4968.
https://digitalcommons.library.tmc.edu/baylor_docs/4968
Included in
Health Services Research Commons, Medical Cell Biology Commons, Medical Molecular Biology Commons, Medical Specialties Commons, Microbiology Commons