Language
English
Publication Date
8-24-2024
Journal
Scientific Data
DOI
10.1038/s41597-024-03628-y
PMID
39181902
PMCID
PMC11344823
PubMedCentral® Posted Date
8-24-2024
PubMedCentral® Full Text Version
Post-print
Abstract
Phlebotomine sand flies are the vectors of leishmaniasis, a neglected tropical disease. High-quality reference genomes are an important tool for understanding the biology and eco-evolutionary dynamics underpinning disease epidemiology. Previous leishmaniasis vector reference sequences were limited by sequencing technologies available at the time and inadequate for high-resolution genomic inquiry. Here, we present updated reference assemblies of two sand flies, Phlebotomus papatasi and Lutzomyia longipalpis. These chromosome-level assemblies were generated using an ultra-low input library protocol, PacBio HiFi long reads, and Hi-C technology. The new P. papatasi reference has a final assembly span of 351.6 Mb and contig and scaffold N50s of 926 kb and 111.8 Mb, respectively. The new Lu. longipalpis reference has a final assembly span of 147.8 Mb and contig and scaffold N50s of 1.09 Mb and 40.6 Mb, respectively. Benchmarking Universal Single-Copy Orthologue (BUSCO) assessments indicated 94.5% and 95.6% complete single copy insecta orthologs for P. papatasi and Lu. longipalpis. These improved assemblies will serve as an invaluable resource for future genomic work on phlebotomine sandflies.
Keywords
Animals, Psychodidae, Genome, Insect, Phlebotomus, Insect Vectors, High-Throughput Nucleotide Sequencing, Sequence Analysis, DNA, Comparative genomics, Genomics, Parasitic infection, Genome assembly algorithms
Published Open-Access
yes
Recommended Citation
Huang, Michelle; Kingan, Sarah; Shoue, Douglas; et al., "Improved High Quality Sand Fly Assemblies Enabled by Ultra Low Input Long Read Sequencing" (2024). Faculty, Staff and Students Publications. 5986.
https://digitalcommons.library.tmc.edu/baylor_docs/5986