Publication Date
7-1-2023
Journal
The Journal of Cardiovascular Aging
DOI
10.20517/jca.2023.18
PMID
37538440
PMCID
PMC10399641
PubMedCentral® Posted Date
8-3-2023
PubMedCentral® Full Text Version
Author MSS
Published Open-Access
yes
Keywords
Atrial remodeling, atrial fibrillation, heart failure, mouse model, transverse aortic constriction
Abstract
INTRODUCTION: Heart failure (HF) is the leading cause of death worldwide. Most large and small animal disease models of HF are based on surgical procedures. A common surgical technique to induce HF is transverse aortic constriction (TAC), which induces pressure overload. The conventional TAC (cTAC) procedure is a highly invasive surgery that is associated with severe inflammation and excessive perioperative deaths.
AIM: To establish an improved, minimally invasive TAC (mTAC) procedure that does not require thoracotomy.
METHODS AND RESULTS: Following anesthesia, mice were intubated, and a small incision was made at the neck and chest. After cutting the sternum about 4 mm, the aortic arch was approached without opening the pleural cavity. A suture was placed between the brachiocephalic artery and the left common carotid artery. This model was associated with low perioperative mortality and a highly reproducible constriction evidenced by an increased right-to-left carotid blood flow velocity ratio in mTAC mice (5.9 ± 0.2)
CONCLUSION: Our data show that the mTAC procedure yields a highly reproducible phenotype consisting of LV contractile dysfunction and enlargement, combined with left atrial enlargement and diastolic dysfunction.
POTENTIAL IMPACT OF THE FINDINGS: This model may be used to test the molecular mechanisms underlying atrial remodeling associated with HF development or to evaluate therapeutic strategies to treat these conditions.
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