Staff and Researcher Publications
Language
English
Publication Date
3-13-2024
Journal
Stem Cell Research & Therapy
DOI
10.1186/s13287-024-03670-y
PMID
38475896
PMCID
PMC10935907
PubMedCentral® Posted Date
3-13-2024
PubMedCentral® Full Text Version
Post-print
Abstract
Background: A traditional view is that stem cells (SCs) divide slowly. Meanwhile, both embryonic and pluripotent SCs display a shorter cell cycle duration (CCD) in comparison to more committed progenitors (CPs).
Methods: We examined the in vitro proliferation and cycling behavior of somatic adult human cells using live cell imaging of passage zero keratinocytes and single-cell RNA sequencing.
Results: We found two populations of keratinocytes: those with short CCD and protracted near exponential growth, and those with long CCD and terminal differentiation. Applying the ergodic principle, the comparative numbers of cycling cells in S phase in an enriched population of SCs confirmed a shorter CCD than CPs. Further, analysis of single-cell RNA sequencing of cycling adult human keratinocyte SCs and CPs indicated a shortening of both G1 and G2M phases in the SC.
Conclusions: Contrary to the pervasive paradigm, SCs progress through cell cycle more quickly than more differentiated dividing CPs. Thus, somatic human adult keratinocyte SCs may divide infrequently, but divide rapidly when they divide. Additionally, it was found that SC-like proliferation persisted in vitro.
Keywords
Adult, Humans, Cell Proliferation, Cell Cycle, Cell Division, Cell Differentiation, Phenotype, Pluripotent Stem Cells, Keratinocytes
Published Open-Access
yes
Recommended Citation
Xiao, Tong; Eze, Ugomma C; Charruyer-Reinwald, Alex; et al., "Short Cell Cycle Duration Is a Phenotype of Human Epidermal Stem Cells" (2024). Staff and Researcher Publications. 16.
https://digitalcommons.library.tmc.edu/clinic_pub/16
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