Center for Medical Ethics and Health Policy Staff Publications
Language
English
Publication Date
1-1-2022
Journal
PLoS One
DOI
10.1371/journal.pone.0275013
PMID
36155987
PMCID
PMC9512193
PubMedCentral® Posted Date
9-26-2022
PubMedCentral® Full Text Version
Post-print
Abstract
Metabolomics provides a powerful tool to study physiological changes in response to various perturbations such as vaccination. We explored whether metabolomic changes could be seen after vaccination in a phase I trial where Gabonese adults living either in rural or semi-urban areas received the subunit hookworm vaccine candidates (Na-GST-1 and Na-APR-1 (M74) adjuvanted with Alhydrogel plus GLA-AF (n = 24) or the hepatitis B vaccine (n = 8) as control. Urine samples were collected and assayed using targeted 1H NMR spectroscopy. At baseline, a set of metabolites significantly distinguished rural from semi-urban individuals. The pre- and post-vaccination comparisons indicated significant changes in few metabolites but only one day after the first vaccination. There was no relationship with immunogenicity. In conclusion, in a small phase 1 trial, urinary metabolomics could distinguish volunteers with different environmental exposures and reflected the safety of the vaccines but did not show a relationship to immunogenicity.
Keywords
Adjuvants, Immunologic, Adult, Aluminum Hydroxide, Ancylostomatoidea, Animals, Gabon, Hepatitis B Vaccines, Hookworm Infections, Humans, Immunogenicity, Vaccine
Published Open-Access
yes
Recommended Citation
Betouke Ongwe, Madeleine Eunice; Mouwenda, Yoanne D; Stam, Koen A; et al., "Investigation of Urinary Metabolomics in a Phase I Hookworm Vaccine Trial in Gabon" (2022). Center for Medical Ethics and Health Policy Staff Publications. 218.
https://digitalcommons.library.tmc.edu/med_ethics/218