Author ORCID Identifier
0000-0002-4020-1822
Date of Graduation
8-2022
Document Type
Dissertation (PhD)
Program Affiliation
Biostatistics, Bioinformatics and Systems Biology
Degree Name
Doctor of Philosophy (PhD)
Advisor/Committee Chair
Nick Navin
Committee Member
Ken Chen
Committee Member
Simona Shaitelman
Committee Member
Cullen Tanaguchi
Committee Member
Jennifer Wargo
Abstract
This work utilizes single cell RNA sequencing to identify transcriptional populations and gene changes for the purpose of immune-related cancer therapies. First we have characterized the T cell populations of the normal and malignant human pancreas. Furthermore, we utilized single cell TCR sequencing to track transcriptional states of T cell clones from human PDACs into a T cell culture product for adoptive cell therapy. Second, we examined the potential role of radiotherapy in inducing an immune response in hormone receptor positive breast tumors.
Keywords
single cell, breast cancer, genomics, PDAC, cell therapy, radiotherapy