Date of Graduation

8-2024

Document Type

Dissertation (PhD)

Program Affiliation

Cancer Biology

Degree Name

Doctor of Philosophy (PhD)

Advisor/Committee Chair

George A. Calin, M.D., Ph.D.

Committee Member

M. James You, M.D., Ph.D.

Committee Member

Marina Konopleva, M.D., Ph.D.

Committee Member

Alessandra Ferrajoli, M.D.

Committee Member

Maria Teresa Bertilaccio, Ph.D.

Committee Member

Yejing Ge, Ph.D.

Abstract

Ultraconserved regions (UCRs) are genomic segments with perfect (100%) conservation between the orthologous regions of human, rat, and mouse genomes. UCRs can be transcribed into mono-exonic long non-coding RNAs (lncRNAs) known as transcribed ultraconserved regions (T-UCRs). These regions, despite lacking protein-coding potential, are increasingly recognized for their regulatory roles in gene expression, including the modulation of non-coding RNA (ncRNA) transcripts. NcRNAs play crucial roles in cellular processes, including oncogenic transformation, with emerging evidence revealing their ability to encode small peptides known as ncRNA-encoded peptides (ncPEPs). These peptides, originating from small open reading frames (smORFs), contribute to diverse cellular functions and are implicated in disease pathogenesis.

Keywords

Functional Peptides, Ultraconserved Regions, CRISPR-Cas9, Chronic Lymphocytic Leukemia, Adaptive Immunity, B-cell Development

Available for download on Friday, July 25, 2025

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