Dissertations and Theses (Open Access)
Author ORCID Identifier
0009-0009-7833-8320
Date of Graduation
8-2025
Document Type
Thesis (MS)
Program Affiliation
Biomedical Sciences
Degree Name
Masters of Science (MS)
Advisor/Committee Chair
M. James You
Committee Member
Pamela L. Wenzel
Committee Member
Angela H. Ting
Committee Member
Shih-Han Lee
Committee Member
George A. Calin
Committee Member
Sean M. Post
Abstract
Cleavage and polyadenylation specific factor 6 (Cpsf6) translocation is frequently reported in APLL, which is Acute Promyelocytic Leukemia (APL)-like acute myeloid leukemia (AML) and other types of blood diseases associated with abnormal hematopoiesis. CPSF6 is a subunit of the Cleavage Factor I mammalian (CFIm) complex, which regulates post-transcriptional alternative polyadenylation (APA) of pre-mRNAs. Previous research indicated that Cpsf6 plays an essential role in alternative polyadenylation, development, and tumorigenesis. However, the role of Cpsf6 in hematopoiesis is unknown. Given its roles in APA, development and cancer, we hypothesize that Cpsf6 plays a critical role in hematopoiesis and that its dysregulation may have significant impacts on this process. To test this, we used conditional knockout mouse models to examine the function of Cpsf6 in both embryonic and adult hematopoiesis. Our findings demonstrate that Cpsf6 is essential for embryonic hematopoiesis but is dispensable for maintaining adult hematopoietic homeostasis.
In addition to this primary work, I contributed to related exploratory studies on RARG fusion genes reported in APLL. My efforts laid the foundation for future work on RARG fusions in leukemia.
Recommended Citation
Ye, Fengxi, "The Role of Cleavage and Polyadenylation Spcific Factor 6 (CPSF6) in Hematopoiesis" (2025). Dissertations and Theses (Open Access). 1469.
https://digitalcommons.library.tmc.edu/utgsbs_dissertations/1469
Keywords
Hematopoiesis, CPSF6, Leukemia, APL, Alternative Polyadenylation