Dissertations and Theses (Open Access)

Author ORCID Identifier

0009-0001-0199-4735

Date of Graduation

8-2026

Document Type

Thesis (MS)

Program Affiliation

Neuroscience

Degree Name

Masters of Science (MS)

Advisor/Committee Chair

Federica Pisaneschi

Committee Member

Andrea Stavoe

Committee Member

Longjun Wu

Committee Member

Niki Millward

Committee Member

Joya Chandra

Abstract

Neuroinflammation, an inflammatory response in the central nervous system (CNS), has been demonstrated to be a key contributor to the pathogenesis and progression of neurological conditions and disorders. During an inflammatory response in the CNS, resident immune cells like microglia become activated, respond to pathological stimuli with a cascade of signaling molecules, engage local repair mechanisms, and can transition into disease-associated microglia (DAM) states through the upregulation of microglia-specific receptors, such as triggering receptor expressed on myeloid cells 2 (TREM2). Although initially protective, sustained inflammatory activation drives synaptic loss, neuronal death, and cognitive and functional deficits. Translatable imaging tools to track microglial activation is therefore essential for both early diagnosis and therapeutic development. To address these challenges, we aim to detect neuroinflammation in two pathological states using a novel positron emission tomography (PET) antibody-based tracer which is radiolabeled with zirconium-89 (89Zr). Because of its characteristics as a bispecific antibody and subsequent ability to target transferrin receptors and selectively bind to TREM2, we hypothesize that we can utilize our tracer, 89Zr-T2BA, as a specific tool for monitoring TREM2 upregulation and ensuing microglial activation across neuroinflammatory models, including lipopolysaccharide (LPS)-induced inflammation and cranial radiotherapy (CRT)-induced injury.

Keywords

TREM2, neuroinflammation, cranial radiotherapy, positron emission tomography, immunoPET

Available for download on Tuesday, December 08, 2026

Share

COinS