Dissertations and Theses (Open Access)
Date of Graduation
12-2010
Document Type
Dissertation (PhD)
Program Affiliation
Biomedical Sciences
Degree Name
Doctor of Philosophy (PhD)
Advisor/Committee Chair
Dr. Lei Li
Committee Member
Dr. Andreas Bergmann
Committee Member
Dr. Randy J. Legerski
Committee Member
Dr. Mong-Hong Lee
Committee Member
Dr. Guillermina Lozano
Abstract
RMI1 (BLM-Associated Protein 75 or Blap75) is highly conserved from yeast to human. Previous studies have shown that hRMI1 is required for BLM/TopoIIIα/RMI1 complex stability and function. However, in vivo functions of RMI1 remain elusive. To address this question, I generated RMI1 knockout mice by homologous replacement targeting. While RMI1+/- mice showed no obvious phenotype, deletion of both RMI1 alleles leads to early embryonic lethality before implantation. I then generated RMI1/p53 double knockout mice. After ionizing radiation treatment at 4Gy, RMI1/p53 double-heterzygous mice showed shortened tumor latency and aggressive tumor types when comparing with wild type, RMI1+/- and p53+/- control cohorts. My study suggests a dual-functional role of RMI1 in early embryonic development and tumor suppression.
Recommended Citation
Chen, Haoyi, "Rmi1 Is Essential For Early Embryonic Development and Attenuation of Tumor Development" (2010). Dissertations and Theses (Open Access). 94.
https://digitalcommons.library.tmc.edu/utgsbs_dissertations/94
Keywords
RMI1, HR, DSB.