A Developmental Stage-Specific Network Approach for Studying Dynamic Co-regulation of Transcription Factors and microRNAs During Craniofacial Development

Authors

Language

English

Publication Date

12-24-2020

Journal

Development

DOI

10.1242/dev.192948

PMID

33234712

PMCID

PMC7774895

PubMedCentral® Posted Date

12-24-2020

PubMedCentral® Full Text Version

Post-print

Abstract

Craniofacial development is regulated through dynamic and complex mechanisms that involve various signaling cascades and gene regulations. Disruption of such regulations can result in craniofacial birth defects. Here, we propose the first developmental stage-specific network approach by integrating two crucial regulators, transcription factors (TFs) and microRNAs (miRNAs), to study their co-regulation during craniofacial development. Specifically, we used TFs, miRNAs and non-TF genes to form feed-forward loops (FFLs) using genomic data covering mouse embryonic days E10.5 to E14.5. We identified key novel regulators (TFs Foxm1, Hif1a, Zbtb16, Myog, Myod1 and Tcf7, and miRNAs miR-340-5p and miR-129-5p) and target genes (

Keywords

Animals, Facial Bones, Forkhead Box Protein M1, Gene Expression Regulation, Neoplastic, Hepatocyte Nuclear Factor 1-alpha, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, Mice, MicroRNAs, MyoD Protein, Myogenin, Promyelocytic Leukemia Zinc Finger Protein, Skull, Transcription Factors, Wnt Signaling Pathway

Published Open-Access

yes

Recommended Citation

, "A Developmental Stage-Specific Network Approach for Studying Dynamic Co-regulation of Transcription Factors and microRNAs During Craniofacial Development" (2020). Faculty, Staff and Student Publications. 76.
https://digitalcommons.library.tmc.edu/uthdb_docs/76