Faculty, Staff and Student Publications
Language
English
Publication Date
7-6-2024
Journal
Journal of Developmental Biology
DOI
10.3390/jdb12030019
PMID
39051201
PMCID
PMC11270360
PubMedCentral® Posted Date
7-6-2024
PubMedCentral® Full Text Version
Post-print
Abstract
Frontonasal malformations are caused by a failure in the growth of the frontonasal prominence during development. Although genetic studies have identified genes that are crucial for frontonasal development, it remains largely unknown how these genes are regulated during this process. Here, we show that microRNAs, which are short non-coding RNAs capable of targeting their target mRNAs for degradation or silencing their expression, play a crucial role in the regulation of genes related to frontonasal development in mice. Using the Mouse Genome Informatics (MGI) database, we curated a total of 25 mouse genes related to frontonasal malformations, including frontonasal hypoplasia, frontonasal dysplasia, and hypotelorism. MicroRNAs regulating the expression of these genes were predicted through bioinformatic analysis. We then experimentally evaluated the top three candidate miRNAs (miR-338-5p, miR-653-5p, and miR-374c-5p) for their effect on cell proliferation and target gene regulation in O9-1 cells, a neural crest cell line. Overexpression of these miRNAs significantly inhibited cell proliferation, and the genes related to frontonasal malformations (
Keywords
microRNA, gene regulatory network, frontonasal malformations, craniofacial development
Published Open-Access
yes
Recommended Citation
, "Genes Related to Frontonasal Malformations Are Regulated by miR-338-5p, miR-653-5p, and miR-374-5p in O9-1 Cells" (2024). Faculty, Staff and Student Publications. 77.
https://digitalcommons.library.tmc.edu/uthdb_docs/77