Protocol for Expression of Murine Milk Using Modified Human Breast Pump Parts

Authors

Cydney Meyer
Joseph L Alcorn

Publication Date

9-15-2023

Journal

Star Protocols

Abstract

Understanding the nutritional and immunomodulatory components of breast milk is crucial to developing novel mechanisms to optimize neonatal health. Here, we present a protocol to express and isolate murine milk in sufficient quantities for further analysis of components and bioactivity. We describe steps for separating dams from pups, administering intraperitoneal anesthetic and oxytocin, and expressing milk using a minimally modified and readily available commercial breast pump parts. For complete details on the use and execution of this protocol, please refer to Meyer et al. (2022).

Keywords

Animals, Female, Mice, Milk, Developmental Biology, Health Sciences, Model Organisms

Comments

PMID: 37516971

DOI

10.1016/j.xpro.2023.102475

PMID

37516971

PMCID

PMC10400948

PubMedCentral® Posted Date

July 2023

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes