Pyridoxamine Treatment Ameliorates Large Artery Stiffening and Cerebral Artery Endothelial Dysfunction in Old Mice

Authors

Emily H Reeve
Elise K Kronquist
Julia R Wolf
Byron Lee
Aleena Khurana
Hanson Pham
Abigail E Cullen
Jessica A Peterson
Antonio Meza
R Colton Bramwell
Laura Villasana
Daniel R Machin
Grant D Henson
Ashley E Walker

Publication Date

2-1-2023

Journal

Journal of Cerebral Blood Flow & Metabolism

Abstract

Age-related increases in large artery stiffness are associated with cerebrovascular dysfunction and cognitive impairment. Pyridoxamine treatment prevents large artery stiffening with advancing age, but the effects of pyridoxamine treatment on the cerebral vasculature or cognition is unknown. The purpose of this study was to investigate the effects of pyridoxamine on blood pressure, large artery stiffness, cerebral artery function, and cognitive function in old mice. Old male C57BL/6 mice consumed either pyridoxamine (2 g/L) or vehicle control in drinking water for ∼7.5 months and were compared with young male C57BL/6 mice. From pre- to post-treatment, systolic blood pressure increased in old control mice, but was maintained in pyridoxamine treated mice. Large artery stiffness decreased in pyridoxamine-treated mice but was unaffected in control mice. Pyridoxamine-treated mice had greater cerebral artery endothelium-dependent dilation compared with old control mice, and not different from young mice. Old control mice had impaired cognitive function; however, pyridoxamine only partially preserved cognitive function in old mice. In summary, pyridoxamine treatment in old mice prevented age-related increases in blood pressure, reduced large artery stiffness, preserved cerebral artery endothelial function, and partially preserved cognitive function. Taken together, these results suggest that pyridoxamine treatment may limit vascular aging.

Keywords

Mice, Male, Animals, Pyridoxamine, Mice, Inbred C57BL, Cerebral Arteries, Aging, Vascular Diseases, Vascular Stiffness, Endothelium, Vascular, Aging, cerebrovascular dysfunction, cognitive impairment, large artery stiffness, pyridoxamine

Comments

Supplementary Materials

PMID: 36189840