SDC1 and ITGA2 As Novel Prognostic Biomarkers for PDAC Related to IPMN

Authors

Chuan-Long Zhang
Qian Shen
Fu-Dong Liu
Fan Yang
Meng-Qi Gao
Xiao-Chen Jiang
Yi Li
Xi-Yuan Zhang
Ge-Er En
Xue Pan
Bo Pang

Publication Date

10-31-2023

Journal

Scientific Reports

Abstract

The existing biomarkers are insufficient for predicting the prognosis of pancreatic ductal adenocarcinoma (PDAC). Intraductal papillary mucinous neoplasm (IPMN) is a precursor to PDAC; therefore, identifying biomarkers from differentially expressed genes (DEGs) of PDAC and IPMN is a new and reliable strategy for predicting the prognosis of PDAC. In this study, four datasets were downloaded from the Gene Expression Omnibus database and standardized using the R package 'limma.' A total of 51 IPMN and 81 PDAC samples were analyzed, and 341 DEGs in PDAC and IPMN were identified; DEGs were involved in the extracellular matrix and tumor microenvironment. An acceptable survival prognosis was demonstrated by SDC1 and ITGA2, which were highly expressed during in vitro PDAC cell proliferation, apoptosis, and migration. SDC1high was enriched in interferon alpha (IFN-α) response and ITGA2high was primarily detected in epithelial-mesenchymal transition (EMT), which was verified using western blotting. We concluded that SDC1 and ITGA2 are potential prognostic biomarkers for PDAC associated with IPMN. Downregulation of SDC1 and ITGA2 expression in PDAC occurs via a mechanism involving possible regulation of IFN-α response, EMT, and immunity, which may act as new targets for PDAC therapy.

Keywords

Humans, Biomarkers, Tumor, Carcinoma, Pancreatic Ductal, Pancreatic Intraductal Neoplasms, Pancreatic Neoplasms, Prognosis, Syndecan-1, Tumor Microenvironment

Comments

Supplementary Material

Data Availability Statement

PMID: 37907515

DOI

10.1038/s41598-023-44646-x

PMID

37907515

PMCID

PMC10618477

PubMedCentral® Posted Date

October 2023

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes