A Potent and Broad Neutralization of SARS-Cov-2 Variants of Concern by DARpins

Authors

Vikas Chonira
Young D Kwon
Jason Gorman
James Brett Case
Zhiqiang Ku
Rudo Simeon
Ryan G Casner
Darcy R Harris
Adam S Olia
Tyler Stephens
Lawrence Shapiro
Michael F Bender
Hannah Boyd
I-Ting Teng
Yaroslav Tsybovsky
Florian Krammer
Ningyan Zhang
Michael S Diamond
Peter D Kwong
Zhiqiang An
Zhilei Chen

Publication Date

3-1-2023

Journal

Nature Chemical Biology

DOI

10.1038/s41589-022-01193-2

PMID

36411391

PMCID

PMC10294592

PubMedCentral® Posted Date

March 2024

PubMedCentral® Full Text Version

Author MSS

Abstract

We report the engineering and selection of two synthetic proteins-FSR16m and FSR22-for the possible treatment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. FSR16m and FSR22 are trimeric proteins composed of DARPin SR16m or SR22 fused with a T4 foldon. Despite selection by a spike protein from a now historical SARS-CoV-2 strain, FSR16m and FSR22 exhibit broad-spectrum neutralization of SARS-CoV-2 strains, inhibiting authentic B.1.351, B.1.617.2 and BA.1.1 viruses, with respective IC

Keywords

Animals, Mice, Humans, SARS-CoV-2, Designed Ankyrin Repeat Proteins, COVID-19, Mice, Transgenic

Published Open-Access

yes

Recommended Citation

Chonira, Vikas; Kwon, Young D; Gorman, Jason; et al., "A Potent and Broad Neutralization of SARS-Cov-2 Variants of Concern by DARpins" (2023). Faculty, Staff and Student Publications. 172.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/172