TRPV4 Expression in the Renal Tubule Is Necessary for Maintaining Whole Body K+ Homeostasis

Authors

Anna Stavniichuk
Kyrylo Pyrshev
Oleg Zaika
Viktor N Tomilin
Mariya Kordysh
Monika Lakk
David Križaj
Oleh Pochynyuk

Publication Date

6-1-2023

Journal

American Journal of Physiology-Renal Physiology

Abstract

The Ca2+-permeable transient receptor potential vanilloid type 4 (TRPV4) channel serves as the sensor of tubular flow, thus being well suited to govern mechanosensitive K+ transport in the distal renal tubule. Here, we directly tested whether the TRPV4 function is significant in affecting K+ balance. We used balance metabolic cage experiments and systemic measurements with different K+ feeding regimens [high (5% K+), regular (0.9% K+), and low (<0.01% K+)] in newly created transgenic mice with selective TRPV4 deletion in the renal tubule (TRPV4fl/fl-Pax8Cre) and their littermate controls (TRPV4fl/fl). Deletion was verified by the absence of TRPV4 protein expression and lack of TRPV4-dependent Ca2+ influx. There were no differences in plasma electrolytes, urinary volume, and K+ levels at baseline. In contrast, plasma K+ levels were significantly elevated in TRPV4fl/fl-Pax8Cre mice on high K+ intake. K+-loaded knockout mice exhibited lower urinary K+ levels than TRPV4fl/fl mice, which was accompanied by higher aldosterone levels by day 7. Moreover, TRPV4fl/fl-Pax8Cre mice had more efficient renal K+ conservation and higher plasma K+ levels in the state of dietary K+ deficiency. H+-K+-ATPase levels were significantly increased in TRPV4fl/fl-Pax8Cre mice on a regular diet and especially on a low-K+ diet, pointing to augmented K+ reabsorption in the collecting duct. Consistently, we found a significantly faster intracellular pH recovery after intracellular acidification, as an index of H+-K+-ATPase activity, in split-opened collecting ducts from TRPV4fl/fl-Pax8Cre mice. In summary, our results demonstrate an indispensable prokaliuretic role of TRPV4 in the renal tubule in controlling K+ balance and urinary K+ excretion during variations in dietary K+ intake.

Keywords

Animals, Mice, Adenosine Triphosphatases, Homeostasis, Hypokalemia, Kidney Tubules, Kidney Tubules, Distal, Mice, Knockout, Mice, Transgenic, Potassium Deficiency, TRPV Cation Channels

Comments

Supplementary Material

Data Availability Statement

PMID: 37141145

DOI

10.1152/ajprenal.00278.2022

PMID

37141145

PMCID

PMC10281785

PubMedCentral® Posted Date

May 2023

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes