Spatially Aware Dimension Reduction for Spatial Transcriptomics

Authors

Lulu Shang
Xiang Zhou

Publication Date

11-23-2022

Journal

Nature Communications

Abstract

Spatial transcriptomics are a collection of genomic technologies that have enabled transcriptomic profiling on tissues with spatial localization information. Analyzing spatial transcriptomic data is computationally challenging, as the data collected from various spatial transcriptomic technologies are often noisy and display substantial spatial correlation across tissue locations. Here, we develop a spatially-aware dimension reduction method, SpatialPCA, that can extract a low dimensional representation of the spatial transcriptomics data with biological signal and preserved spatial correlation structure, thus unlocking many existing computational tools previously developed in single-cell RNAseq studies for tailored analysis of spatial transcriptomics. We illustrate the benefits of SpatialPCA for spatial domain detection and explores its utility for trajectory inference on the tissue and for high-resolution spatial map construction. In the real data applications, SpatialPCA identifies key molecular and immunological signatures in a detected tumor surrounding microenvironment, including a tertiary lymphoid structure that shapes the gradual transcriptomic transition during tumorigenesis and metastasis. In addition, SpatialPCA detects the past neuronal developmental history that underlies the current transcriptomic landscape across tissue locations in the cortex.

Keywords

Transcriptome/, Gene Expression Profiling, Genome, Exome Sequencing, Tumor Microenvironment, Statistical methods, Computational models, Transcriptomics, Tumour heterogeneity, Cancer microenvironment

Comments

Associated Data

Data availability

PMID: 36418351