Faculty, Staff and Student Publications
Publication Date
8-4-2023
Journal
Nature Communications
Abstract
The SWI/SNF ATP-dependent chromatin remodeler is a master regulator of the epigenome, controlling pluripotency and differentiation. Towards the C-terminus of the catalytic subunit of SWI/SNF is a motif called the AT-hook that is evolutionary conserved. The AT-hook is present in many chromatin modifiers and generally thought to help anchor them to DNA. We observe however that the AT-hook regulates the intrinsic DNA-stimulated ATPase activity aside from promoting SWI/SNF recruitment to DNA or nucleosomes by increasing the reaction velocity a factor of 13 with no accompanying change in substrate affinity (K
Keywords
Animals, Mice, Transcription Factors, Saccharomyces cerevisiae, Cell Lineage, Chromatin, Nucleosomes, DNA, Adenosine Triphosphate
DOI
10.1038/s41467-023-40386-8
PMID
37542049
PMCID
PMC10403523
PubMedCentral® Posted Date
August 2023
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Included in
Bioinformatics Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Biomedical Informatics Commons, Medical Microbiology Commons, Medical Molecular Biology Commons, Medical Specialties Commons
Comments
Supplementary Material
PMID: 37542049