Predictive Capacity of a miRNA Panel in Identifying Teratoma in Post-Chemotherapy Consolidation Surgeries

Authors

Joseph A Moore
Michael J Lehner
Simone Anfossi
Saumil Datar
Rebecca S Tidwell
Matthew Campbell
Amishi Y Shah
John F Ward
Jose A Karam
Christopher G Wood
Lois L Pisters
George A Calin
Shi-Ming Tu

Publication Date

1-1-2023

Journal

BJUI Compass

DOI

10.1002/bco2.143

PMID

36569509

PMCID

PMC9766861

PubMedCentral® Posted Date

7-5-2022

PubMedCentral® Full Text Version

Post-print

Abstract

OBJECTIVES: To investigate the utility of a novel serum miRNA biomarker panel to distinguish teratoma from nonmalignant necrotic/fibrotic tissues or nonviable tumours in patients with NSGCT undergoing post-chemotherapy consolidation surgery.

PATIENTS AND METHODS: We prospectively collected pre-surgical serum samples from 22 consecutive testicular NSGCT patients with residual NSGCT after chemotherapy undergoing post-chemotherapy consolidation surgery. We measured serum miRNA expression of four microRNAs (miRNA-375, miRNA-200a-3p, miRNA-200a-5p and miRNA-200b-3p) and compared with pathologic findings at time of surgery. Receiver operating characteristic (ROC) curves were performed to assess the ability of these miRNA to differentiate between teratoma and necrosis or viable malignancy.

RESULTS: Twenty-two patients with NSGCT were split into two groups based on pathology at time of post-chemotherapy consolidation surgery (teratoma group vs. necrosis/fibrosis/viable tumour group, i.e., NFVT). Patients with teratoma were older at diagnosis compared with those patients with NFVT (median age 28.7 vs. 23.9). Patients with NFVT were more likely to have embryonal carcinoma in their primary tumour (81.8% vs. 27.3%;

CONCLUSION: This novel miRNA panel (miRNA-375, miRNA-200a-3p, miRNA-200a-5p and miRNA-200b-3p) did not distinguish teratoma from nonmalignant necrotic/fibrotic tissues or nonviable tumours in patients with NSGCT undergoing post-chemotherapy consolidation surgery.

Keywords

biomarker, micro RNA, miRNA, non‐seminomatous germ cell tumour, teratoma

Published Open-Access

yes

Recommended Citation

Moore, Joseph A; Lehner, Michael J; Anfossi, Simone; et al., "Predictive Capacity of a miRNA Panel in Identifying Teratoma in Post-Chemotherapy Consolidation Surgeries" (2023). Faculty, Staff and Student Publications. 2689.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/2689