Generation of a Wt1 Conditional Deletion, Nuclear Red Fluorescent Protein Reporter Allele in the Mouse

Authors

Jace A Aloway
E Cristy Ruteshouser
Vicki Huff
Richard R Behringer

Publication Date

1-1-2024

Journal

Differentiation

DOI

10.1016/j.diff.2024.100791

PMID

38941819

PMCID

PMC12118357

PubMedCentral® Posted Date

7-1-2025

PubMedCentral® Full Text Version

Author MSS

Abstract

A Wt1 conditional deletion, nuclear red fluorescent protein (RFP) reporter allele was generated in the mouse by gene targeting in embryonic stem cells. Upon Cre-mediated recombination, a deletion allele is generated that expresses RFP in a Wt1-specific pattern. RFP expression was detected in embryonic and adult tissues known to express Wt1, including the kidney, mesonephros, and testis. In addition, RFP expression and WT1 co-localization was detected in the adult uterine stroma and myometrium, suggesting a role in uterine function. Crosses with Wnt7a-Cre transgenic mice that express Cre in the Müllerian duct epithelium activate Wt1-directed RFP expression in the epithelium of the oviduct but not the stroma and myometrium of the uterus. This new mouse strain should be a useful resource for studies of Wt1 function and marking Wt1-expressing cells.

Keywords

Animals, Mice, Red Fluorescent Protein, WT1 Proteins, Luminescent Proteins, Alleles, Mice, Transgenic, Female, Genes, Reporter, Male, Gene Deletion, Wilms’ tumor, kidney, testis, uterus, oviduct, mesonephros

Published Open-Access

yes

Recommended Citation

Aloway, Jace A; Ruteshouser, E Cristy; Huff, Vicki; et al., "Generation of a Wt1 Conditional Deletion, Nuclear Red Fluorescent Protein Reporter Allele in the Mouse" (2024). Faculty, Staff and Student Publications. 4248.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/4248