Faculty, Staff and Student Publications
Publication Date
6-1-2025
Journal
Cancer Research Communications
DOI
10.1158/2767-9764.CRC-23-0189
PMID
40377974
PMCID
PMC12163576
PubMedCentral® Posted Date
6-13-2025
PubMedCentral® Full Text Version
Post-print
Abstract
Glioblastoma (GBM) is the most common and deadly primary brain malignancy and is clinically refractory to immunotherapy. Active NLRP3 inflammasome signaling and IL-1β secretion have been observed in GBM, and NLRP3-driven myeloid-derived suppressor cell (MDSC) recruitment can mediate cancer immune evasion. Agonists of the cytosolic double-stranded DNA-sensing stimulator of IFN gene (STING) pathway can mediate proinflammatory conversion of cancer MDSCs; however, secretion of the NLRP3 products IL-1β and IL-18 has also been observed in certain myeloid populations following STING activation. In this study, we aimed to determine both the potential mechanistic synergy between STING and NLRP3 agonists, and the effects of this innate immune combination on the GBM tumor immune landscape. We find that STING activation does not prime pro-IL-1β expression for activated NLRP3 inflammasome secretion. In subcutaneous GL261 GBM, we show that NLRP3 activation expands the immunosuppressive myeloid stroma primarily via granulocytic MDSC recruitment and antagonizes the benefit of STING activation. In brain GL261, we find that NLRP3 activation expands granulocytic MDSCs but does not antagonize the therapeutic benefit of STING activation. Finally, we report that mesenchymal subtype GBM tumors have elevated neutrophil, IL-1β, and NLRP3 gene expression, a setting where our data suggest that NLRP3 activation could counteract STING agonists.
Significance: NLRP3 inflammasome signaling, which suppresses antitumor immunity in some cancers, has been observed in GBM tissues. NLRP3 activation in GBM induces granulocyte-dependent tumor immunosuppression and antagonizes the therapeutic efficacy of STING activation.
Keywords
Glioblastoma, NLR Family, Pyrin Domain-Containing 3 Protein, Inflammasomes, Animals, Membrane Proteins, Mice, Brain Neoplasms, Myeloid-Derived Suppressor Cells, Humans, Cell Line, Tumor, Mice, Inbred C57BL, Interleukin-1beta
Published Open-Access
yes
Recommended Citation
Lea, Spencer T; Chen, Chao-Hsien; Wei, Jun; et al., "NLRP3 Inflammasome Activation Expands the Immunosuppressive Myeloid Stroma and Antagonizes the Therapeutic Benefit of STING Activation in Glioblastoma" (2025). Faculty, Staff and Student Publications. 4320.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/4320
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