Faculty, Staff and Student Publications
Publication Date
7-1-2025
Journal
STAR Protocols
DOI
10.1016/j.xpro.2025.103924
PMID
40601427
PMCID
PMC12268536
PubMedCentral® Posted Date
7-1-2025
PubMedCentral® Full Text Version
Post-print
Abstract
tRNA modifications are highly dynamic and play crucial roles in regulating gene expression. Here, we present an assay for analyzing N2, N2-dimethylguanosine (m22G) modifications on tRNAs using an antibody-based detection approach. We describe steps for isolating tRNAs from human cells via ion-exchange chromatography, capturing specific tRNA iso-decoders on streptavidin magnetic beads, and detecting modifications through immuno-northern blotting. This approach enables researchers to investigate other tRNA modifications using specific antibodies or downstream approaches such as mass spectrometry.
Keywords
Cell Biology, Molecular Biology, Molecular/Chemical Probes
Published Open-Access
yes
Recommended Citation
Sseu-Pei Hwang, Katherine Barondeau, and Catherine Denicourt, "Protocol To Detect m22G Modification on Single tRNA Iso-Decoders by Immuno-Northern Blotting" (2025). Faculty, Staff and Student Publications. 4339.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/4339
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