Faculty, Staff and Student Publications
Publication Date
4-1-2024
Journal
Acta Biomaterialia
DOI
10.1016/j.actbio.2024.02.025
PMID
38387748
PMCID
PMC12016311
PubMedCentral® Posted Date
4-23-2025
PubMedCentral® Full Text Version
Author MSS
Abstract
Bone metastases are the most common milestone in the lethal progression of prostate cancer and prominent in a substantial portion of renal malignancies. Interactions between cancer and bone host cells have emerged as drivers of both disease progression and therapeutic resistance. To best understand these central host-epithelial cell interactions, biologically relevant preclinical models are required. To achieve this goal, we here established and characterized tissue-engineered bone mimetic environments (BME) capable of supporting the growth of patient-derived xenograft (PDX) cells, ex vivo and in vivo. The BME consisted of a polycaprolactone (PCL) scaffold colonized by human mesenchymal stem cells (hMSCs) differentiated into osteoblasts. PDX-derived cells were isolated from bone metastatic prostate or renal tumors, engineered to express GFP or luciferase and seeded onto the BMEs. BMEs supported the growth and therapy response of PDX-derived cells, ex vivo. Additionally, BMEs survived after in vivo implantation and further sustained the growth of PDX-derived cells, their serial transplant, and their application to study the response to treatment. Taken together, this demonstrates the utility of BMEs in combination with patient-derived cells, both ex vivo and in vivo. STATEMENT OF SIGNIFICANCE: Our tissue-engineered BME supported the growth of patient-derived cells and proved useful to monitor the therapy response, both ex vivo and in vivo. This approach has the potential to enable co-clinical strategies to monitor bone metastatic tumor progression and therapy response, including identification and prioritization of new targets for patient treatment.
Keywords
Male, Humans, Xenograft Model Antitumor Assays, Bone and Bones, Bone Neoplasms, Prostatic Neoplasms, Osteoblasts
Published Open-Access
yes
Recommended Citation
Paindelli, Claudia; Parietti, Vanessa; Barrios, Sergio; et al., "Bone Mimetic Environments Support Engineering, Propagation, and Analysis of Therapeutic Response of Patient-Derived Cells, Ex Vivo and In Vivo" (2024). Faculty, Staff and Student Publications. 4360.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/4360
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