Faculty, Staff and Student Publications

Publication Date

9-11-2023

Journal

Developmental Cell

DOI

10.1016/j.devcel.2023.07.025

PMID

37625403

PMCID

PMC10810082

PubMedCentral® Posted Date

9-11-2024

PubMedCentral® Full Text Version

Author MSS

Abstract

Oncogenic KrasG12D (Kras*) is critical for the initiation and maintenance of pancreatic ductal adenocarcinoma (PDAC), and a known repressor of tumor immunity. Conditional elimination of Kras* in genetic mouse models of PDAC leads to reactivation of Fas, CD8+ T cell mediated apoptosis, and complete eradication of tumors. Kras* elimination recruits activated CD4+ and CD8+ T cells and promotes the activation of antigen presenting cells. Mechanistically, Kras* mediated immune evasion involves epigenetic regulation of the Fas death receptor in cancer cells, via methylation of its promoter region. Further, analysis of human RNA sequencing identifies that high KRAS expressing PDAC tumors show a lower proportion of CD8+ T cells and demonstrate shorter survival compared to patients with low KRAS expression. This study highlights the role of CD8+ T cells in eradication of PDAC following Kras* elimination and provides rationale for combination of Kras* targeting with immunotherapy to control PDAC

Keywords

Animals, Humans, Mice, Apoptosis, Carcinoma, Pancreatic Ductal, CD8-Positive T-Lymphocytes, Epigenesis, Genetic, Pancreatic Neoplasms, Proto-Oncogene Proteins p21(ras)

Published Open-Access

yes

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