Faculty, Staff and Student Publications
Publication Date
8-1-2022
Journal
Cell Biology and Toxicology
DOI
10.1007/s10565-022-09698-1
PMID
35267148
PMCID
PMC8907399
PubMedCentral® Posted Date
3-10-2022
PubMedCentral® Full Text Version
Post-print
Abstract
Human lung organoids (hLOs) are useful for disease modelling and drug screening. However, a lack of immune cells in hLOs limits the recapitulation of in vivo cellular physiology. Here, we generated hLOs containing alveolar macrophage (AMφ)-like cells derived from pluripotent stem cells (PSC). To bridge hLOs with advanced human lung high-resolution X-ray computed tomography (CT), we acquired quantitative micro-CT images. Three hLO types were observed during differentiation. Among them, alveolar hLOs highly expressed not only lung epithelial cell markers but also AMφ-specific markers. Furthermore, CD68
Keywords
Alveolar Epithelial Cells, Bleomycin, Humans, Lung, Macrophages, Alveolar, Organoids, Pluripotent Stem Cells, Pulmonary Fibrosis, X-Ray Microtomography
Published Open-Access
yes
Recommended Citation
Choi, Seri; Choi, Jiwoong; Cheon, Seongmin; et al., "Pulmonary Fibrosis Model Using Micro-Ct Analyzable Human Psc-Derived Alveolar Organoids Containing Alveolar Macrophage-Like Cells" (2022). Faculty, Staff and Student Publications. 4585.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/4585
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