An Enzyme-Linked Immunosorbent Assay (ELISA)-Based Activity Assay for AMP-Activated Protein Kinase (AMPK)

Authors

Trezze P Nguyen
Shangze Lyu
Yang Liu

Publication Date

6-1-2025

Journal

FEBS Open Bio

DOI

10.1002/2211-5463.70017

PMID

40066852

PMCID

PMC12127874

PubMedCentral® Posted Date

3-11-2025

PubMedCentral® Full Text Version

Post-print

Abstract

AMP-activated protein kinase (AMPK) is the master regulator of cellular and organismal energy homeostasis, playing an essential role in modulating metabolism and other cellular processes. Substantial efforts have been made to develop pharmacological modulators of AMPK activity due to their therapeutic potential against various diseases. Measuring AMPK activity in vitro is a fundamental step for testing AMPK activators and inhibitors. Here, we report an enzyme-linked immunosorbent assay (ELISA)-based AMPK activity assay with simple steps and high sensitivity. This assay offers a robust, in-house alternative to the traditional radioactive methods and other approaches that rely on specialized reagents or commercial kits.

Keywords

AMP-Activated Protein Kinases, Enzyme-Linked Immunosorbent Assay, Humans, AMPK, ELISA, kinase activity

Published Open-Access

yes

Recommended Citation

Trezze P Nguyen, Shangze Lyu, and Yang Liu, "An Enzyme-Linked Immunosorbent Assay (ELISA)-Based Activity Assay for AMP-Activated Protein Kinase (AMPK)" (2025). Faculty, Staff and Student Publications. 4761.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/4761