Faculty, Staff and Student Publications
Publication Date
6-20-2025
Journal
STAR Protocols
DOI
10.1016/j.xpro.2025.103806
PMID
40327511
PMCID
PMC12138424
PubMedCentral® Posted Date
5-5-2025
PubMedCentral® Full Text Version
Post-print
Abstract
The omentum is a visceral adipose tissue that undergoes dynamic immunological changes prior to and following metastasis. Here, we present a protocol for assessing the mobilization of peritoneal B cells to the pre-metastatic omentum in a mouse ovarian cancer model. We describe steps for isolation and adoptive transfer of peritoneal donor B cells and their detection in the omentum of recipient mice. This protocol could be utilized to study the mobilization of peritoneal B cells to the omentum in other pathological contexts. For complete details on the use and execution of this protocol, please refer to Lee et al.
Keywords
Animals, Female, Omentum, Ovarian Neoplasms, Mice, B-Lymphocytes, Disease Models, Animal, Adoptive Transfer, Peritoneum, Humans, Peritoneal Neoplasms, Cancer, Immunology, Model Organisms
Published Open-Access
yes
Recommended Citation
WonJae Lee, Hironari Akasaka, and Honami Naora, "Protocol for Assessing Mobilization of Peritoneal B Cells to the Pre-Metastatic Omentum in an Orthotopic Mouse Model of Ovarian Cancer" (2025). Faculty, Staff and Student Publications. 4879.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/4879
Graphical Abstract
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