Faculty, Staff and Student Publications
Publication Date
6-20-2025
Journal
STAR Protocols
DOI
10.1016/j.xpro.2025.103911
PMID
40543035
PMCID
PMC12221707
PubMedCentral® Posted Date
6-20-2025
PubMedCentral® Full Text Version
Post-print
Abstract
Lung endothelial cells (LuECs) play crucial roles in lung regeneration and support lung organoids (LOs). However, detailed guidelines for LO culture using LuECs have not been reported yet. Here, we present an efficient protocol for generating and culturing murine LOs that mimic regeneration. We describe steps for the isolation of LuECs and lung epithelial cells, followed by LO culture. We also describe how to establish genetically engineered LOs using viral transduction. For complete details on the use and execution of this protocol, please refer to Kim et al.
Keywords
Cell Biology, Cell culture, Model Organisms, Molecular Biology, Organoids
Published Open-Access
yes
Recommended Citation
Bongjun Kim and Jae-Il Park, "Protocol for Establishing Genetically Engineered Murine Lung Organoids Mimicking Cell Plasticity and Regeneration" (2025). Faculty, Staff and Student Publications. 4949.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/4949
Graphical Abstract
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