Blood and Neuronal Extracellular Vesicle Mitochondrial Disruptions in Schizophrenia

Authors

A Ankeeta
Ashutosh Tripathi
Bindu Pillai
Yizhou Ma
Joshua J Chiappelli
Jessica N Jernberg
Keiko Kunitoki
Xiaoming Du
Si Gao
Bhim M Adhikari
Consuelo Walss-Bass
Giselli Scaini
Peter Kochunov
Anilkumar Pillai
L Elliot Hong

Publication Date

8-23-2025

Journal

Neuropsychopharmacology

DOI

10.1038/s41386-025-02204-1

PMID

40849540

Abstract

The high energy demand of the human brain obligates robust mitochondrial energy metabolism, while mitochondrial dysfunctions have been linked to neuropsychiatric disorders, including schizophrenia spectrum disorders (SSD). However, in vivo assessments that can directly inform brain mitochondrial functioning and its etiopathophysiological path to SSD remain difficult to obtain. We hypothesized that system and brain mitochondrial dysfunctions in SSD may be indexed by elevated cell-free mitochondrial DNA (cf-mtDNA) levels in the blood and in neuronal extracellular vesicles (nEVs). We also explored if these mtDNA marker elevations were associated with brain metabolites as measured by magnetic resonance spectroscopy (MRS). We examined blood cf-mtDNA in 58 SSD patients and 33 healthy controls, followed by assessing nEV mtDNA and metabolite levels using MRS in a subgroup of patients and controls. We found that people with SSD had significantly elevated cf-mtDNA levels in both the blood (p = 0.0002) and neuronal EVs (p = 0.003) compared to controls. These mtDNA abnormalities can be linked back to brain lactate+ levels such that higher blood and nEV mtDNA levels were significantly associated with higher lactate+ levels measured at the anterior cingulate cortex (r = 0.53, 0.53; p = 0.008, 0.03, respectively) in SSD patients. Furthermore, higher developmental stress and trauma were significantly associated with higher cf-mtDNA levels in both the blood and neuronal EVs in SSD patients (r = 0.29, 0.49; p = 0.01, 0.03, respectively). In conclusion, if replicated and fully developed, blood and neuronal EV-based cell-free mtDNA may provide a clinically accessible biomarker to more directly evaluate the mitochondrial hypothesis and the abnormal bioenergetics pathways in schizophrenia.

Published Open-Access

yes

Recommended Citation

Ankeeta, A; Tripathi, Ashutosh; Pillai, Bindu; et al., "Blood and Neuronal Extracellular Vesicle Mitochondrial Disruptions in Schizophrenia" (2025). Faculty, Staff and Student Publications. 4972.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/4972