Faculty, Staff and Student Publications

Publication Date

3-1-2023

Journal

Advanced Science

DOI

10.1002/advs.202204544

PMID

36658690

PMCID

PMC10015900

PubMedCentral® Posted Date

1-19-2023

PubMedCentral® Full Text Version

Post-print

Abstract

Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC-dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics-based single-cell migration platform is described that enables high-throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators.

Keywords

Dendritic Cells, Cell Movement, Adaptive Immunity, Microfluidics, Immunotherapy, cell retrieval, dendritic cell, microfluidics, RNA‐seq, single cell migration

Published Open-Access

yes

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