Faculty, Staff and Student Publications
Publication Date
6-1-2023
Journal
Food Science and Nutrition
DOI
10.1002/fsn3.3339
PMID
37324860
PMCID
PMC10261767
PubMedCentral® Posted Date
4-5-2023
PubMedCentral® Full Text Version
Post-print
Abstract
Commiphora gileadensis (C. gileadensis) has been identified and linked with various health benefits and pharmaceutical potential for its phytochemical activities and chemical constituents. This study aimed to evaluate ultrasonic‐assisted extraction (USE) technique for total phenols content from C. gileadensis leaf compared to the hydrodistillation extraction (HDE). Our results showed that the USE operating conditions were identified as: MeOH·H2O solvent‐to‐fresh sample ratio of 80:20 (v/v); ultrasonic power/frequency at 150 W/20 kHz; and a temperature of 40 ± 1°C; subjected to acoustic waves intermittently for a calculated time (5 min) during the total programmed time of 12 min. The USE exhibited (118.71 ± 0.009 mg GAE/g DM) more amounts of all phenols than HDE (101.47 ± 0.005 mg GAE/g DM), and antioxidant (77.78 ± 0.73%, 75.27 ± 0.59% scavenging inhibition of DPPH), respectively. Anti‐aging and Cytotoxicity activities were investigated. The results of biological evaluations showed that the crude extracts of C. gileadensis significantly extended the replicative lifespan of K6001 yeast. In addition, in vitro cytotoxicity against the HepG2 cell line showed significant anticancer activity, and approximately 100 μg/mL is required to decrease viability compared with that of the control. This study is proven for a larger scale to extract and isolate compounds of C. gileadensis for potential utilization in the pharmaceutical industry. In conclusion, advanced methods afford an extract with high activity in the biological properties of the extract.
Keywords
anti‐aging, Commiphora gileadensis, extraction, HepG2 cells, hydrodistillation, ultrasonic assisted
Published Open-Access
yes
Recommended Citation
Ahmed, Hani; Rashed, Marwan M A; Almoiliqy, Marwan; et al., "Antioxidant Activity and Total Phenolic Compounds of Commiphora gileadensis Extracts Obtained by Ultrasonic-Assisted Extraction, With Monitoring Antiaging and Cytotoxicity Activities" (2023). Faculty, Staff and Student Publications. 5117.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/5117
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