Faculty, Staff and Student Publications

Language

English

Publication Date

1-16-2026

Journal

iScience

DOI

10.1016/j.isci.2025.114289

PMID

41536977

PMCID

PMC12796006

PubMedCentral® Posted Date

11-29-2025

PubMedCentral® Full Text Version

Post-print

Abstract

Acute myeloid leukemia (AML) is a clonal disorder characterized by immature blasts and arrested differentiation that primarily affects the bone marrow (BM) and occasionally presents as extramedullary (EM) disease. EM manifestations highlight AML's adaptability to distinct microenvironments, which we examined using spatial analyses of medullary and EM tissues. We describe a workflow for Visium-based spatial transcriptomics in medullary and EM AML, revealing insights into cell-cell communication and the spatial organization of AML hierarchies. In BM, monocytes and granulocyte-monocyte progenitors colocalized with leukemic populations, sharing molecular signatures with those in EM sample. CXCL12-CXCR4-mediated communication correlated with PI3K/AKT/mTOR signaling in inflammatory niches.

Keywords

Components of the immune system, Proteomics, Transcriptomics

Published Open-Access

yes

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