Protocol for Isolation of Lipid Droplets and Mitochondria From Acute Myeloid Leukemia Cells for Lipidomic Analysis

Authors

Ran Zhao
Liang Zhang
Shuxin Dong
Kazuharu Kamachi
Saurabh Kumar Gupta
Hiroki Akiyama
Jalen Nguyen
G Lavender Hackman
Michael Andreeff
Alessia Lodi
Jo Ishizawa

Language

English

Publication Date

12-15-2025

Journal

STAR Protocols

DOI

10.1016/j.xpro.2025.104257

PMID

41420867

PMCID

PMC12766406

PubMedCentral® Posted Date

12-15-2025

PubMedCentral® Full Text Version

Post-print

Abstract

Lipid droplets (LDs) are dynamic organelles critical for lipid and energy homeostasis. LD dysregulation is implicated in various diseases, including acute myeloid leukemia (AML), which exhibits aberrant lipid metabolism and chemoresistance. Here, we describe an effective protocol for the co-isolation of LDs and mitochondria from lipid-deficient cell types, e.g., AML cells. We detail procedures for the isolation, quality verification, and results from mass spectrometry-based lipidomic analysis. This protocol enables the functional study of organellar lipidomic regulation in AML.

Keywords

cell biology, cell isolation, cell separation/fractionation, mass cytometry, cancer

Published Open-Access

yes

Recommended Citation

Zhao, Ran; Zhang, Liang; Dong, Shuxin; et al., "Protocol for Isolation of Lipid Droplets and Mitochondria From Acute Myeloid Leukemia Cells for Lipidomic Analysis" (2025). Faculty, Staff and Student Publications. 5279.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/5279