Faculty, Staff and Student Publications

Language

English

Publication Date

10-16-2025

Journal

STAR Protocols

DOI

10.1016/j.xpro.2025.104133

PMID

41105517

PMCID

PMC12554166

PubMedCentral® Posted Date

10-16-2025

PubMedCentral® Full Text Version

Post-print

Abstract

Peritoneal carcinomatosis (PC) cells and extracellular vesicles (EVs) from gastric cancer ascites are valuable for studying tumor-stroma interactions. Here, we present a protocol for isolating PC cells and EVs from patient ascites. We describe steps for PC cell culture, iodixanol-based EV purification, electron microscopy, nanoparticle tracking analysis, flow cytometry, and proteomic profiling. This protocol also includes TD-139 loading into exosomes for functional assays to evaluate their role in modulating the tumor microenvironment. For complete details on the use and execution of this protocol, please refer to Fan et al.

Keywords

Cell isolation, Cancer, Proteomics

Published Open-Access

yes

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