Faculty, Staff and Student Publications
Language
English
Publication Date
2-5-2026
Journal
Nature Communications
DOI
10.1038/s41467-025-67110-y
PMID
41644522
Abstract
The exact biological role of mitochondrial supercomplexes remains debated, particularly their role in guiding redox proteins across membranes during energy conversion. We integrate multiscale modeling and single particle cryo-electron microscopy (cryo-EM) to examine electron transfer in mitochondrial supercomplexes composed of complexes III and IV (CIII and CIV). Using bioinformatic and entropy-based methods, we generated structural ensembles capturing conformations of CIII's disordered QCR6 hinge within the yeast CIII2CIV2 supercomplex. Molecular and Brownian Dynamics simulations reveal that these negatively charged hinge states electrostatically couple with redox proteins, promoting their binding and directional diffusion across the membrane on millisecond timescales. Rather than hindering transfer, disorder lowers the diffusion barrier. Anionic lipids reinforce this recognition by retaining a membrane pool of redox proteins when hinge length is critical. Cryo-EM models of ΔQCR6 show large rearrangements, yet maintain a robust electrostatic environment enabling surface-mediated transfer despite reduced charge. Overall, electron carriers confined on bioenergetic membranes follow a refolding-guided diffusion mechanism that enhances supercomplex energy conversion efficiency by nearly 30%.
Published Open-Access
yes
Recommended Citation
Chan, Chun Kit; Nguyen, Jonathan; Hryc, Corey F; et al., "Transient Protein Structure Guides Surface Diffusion Pathways for Electron Transport in Membrane Supercomplexes" (2026). Faculty, Staff and Student Publications. 5603.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/5603
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