Development and Characterization of Orthotopic Patient-Derived Xenograft Models of Peritoneal Metastatic Mucinous Appendiceal Adenocarcinoma

Authors

Ichiaki Ito
Vinay K Pattalachinti
Abdelrahman Mg Yousef
Saikat Chowdhury
Mohammad M Fanaeian
Emaan Haque
Betul Beyza Gunes
Mahmoud Yousef
Emma R Salle
Mohammad A Zeineddine
Shuangxi Ji
Ruonan Li
Wenyi Wang
Beth A Helmink
Melissa W Taggart
Michael G White
Keith F Fournier
Natalie W Fowlkes
John Paul Shen

Language

English

Publication Date

3-1-2025

Journal

ESMO Gastrointestinal Oncology

DOI

10.1016/j.esmogo.2025.100133

PMID

41069644

PMCID

PMC12507331

PubMedCentral® Posted Date

1-31-2025

PubMedCentral® Full Text Version

Post-print

Abstract

Background: Appendiceal adenocarcinomas (AAs) are a rare and heterogeneous group of tumors for which few preclinical models exist. The lack of preclinical models of AA has hindered drug development and is a major factor in why AA remains without a single FDA-approved systemic treatment.

Materials and methods: Tumors from 16 patients with appendiceal neoplasms (15 AA and one HAMN) were implanted into flank and peritoneal cavity of immunodeficient mice leading to the successful establishment of 3 AAPDX models. Histological, immunohistochemical, genetic, and transcriptomic comparison of patient and PDX tumors were performed.

Results: Higher tumor grade, peritoneal implantation, and RAS/RAF mutation were associated with successful tumor engraftment. Comparison of histological, immunohistochemical and molecular analyses including both RNA and DNA sequencing revealed that the PDX models recreate many of the features of metastatic AA, but also displayed several differences between paired PDX and human tumors highlighting the intra-tumoral heterogeneity of AA within each patient. Notably tumor from two patients with primarily low-grade mucinous adenocarcinoma converted to high-grade histology in PDX. Transcriptomic comparison of patient and PDX tumors identified increased Myc and E2F signaling, suggesting that activation of Myc may be a driver of de-differentiation in AA. The established PDX were able to undergo serial passaging and expansion and exhibited stable histological features during this process, allowing for drug testing.

Conclusions: These molecularly profiled, orthotopic PDX models of metastatic AA represent a unique resource for future exploration to identify novel therapies for this orphan disease.

Keywords

appendiceal adenocarcinoma, patient-derived xenograft model, Myc

Published Open-Access

yes

Recommended Citation

Ito, Ichiaki; Pattalachinti, Vinay K; Yousef, Abdelrahman Mg; et al., "Development and Characterization of Orthotopic Patient-Derived Xenograft Models of Peritoneal Metastatic Mucinous Appendiceal Adenocarcinoma" (2025). Faculty, Staff and Student Publications. 5643.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/5643