Protocol for Establishing and Evaluating a Cancer Cachexia Mouse Model

Authors

Zhijun Zhou
Jingxuan Yang
Mingyang Liu
Yu Ren
Xiuhui Shi
Yang Cai
Alex X Arreola
Yi-Ping Li
Yuqing Zhang
Min Li

Language

English

Publication Date

9-20-2024

Journal

STAR Protocols

DOI

10.1016/j.xpro.2024.103281

PMID

39217610

PMCID

PMC11403044

PubMedCentral® Posted Date

8-31-2024

PubMedCentral® Full Text Version

Post-print

Abstract

Cancer cachexia mouse models are needed to recapitulate the clinical features of patients with cachexia. Here, we present a protocol for the establishment and evaluation of cancer cachexia mouse models. We delineate the steps in preparing tumor cells for inoculation and surgical procedures. After the establishment of these mouse models, we describe essential techniques to assess cancer cachexia, including grip strength evaluation, tissue collection, and the calculation of cross-sectional areas of muscle tissue. For complete details on the use and execution of this protocol, please refer to Liu et al.,

Keywords

Cachexia, Animals, Mice, Disease Models, Animal, Neoplasms, Muscle, Skeletal, Cell Biology, Cell culture, Cancer, Health Sciences, Metabolism, Model Organisms, Systems biology

Published Open-Access

yes

Recommended Citation

Zhou, Zhijun; Yang, Jingxuan; Liu, Mingyang; et al., "Protocol for Establishing and Evaluating a Cancer Cachexia Mouse Model" (2024). Faculty, Staff and Student Publications. 6121.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/6121