Tbr2-Dependent Parallel Pathways Regulate the Development of Distinct ipRGC Subtypes

Authors

Takae Kiyama
Ching-Kang Chen
Halit Y Altay
Yu-Jiun Chen
Leviette Sigala
Dan Su
Steven Eliason
Brad A Amendt
Chai-An Mao

Language

English

Publication Date

2-1-2026

Journal

Communications Biology

DOI

10.1038/s42003-026-09645-4

PMID

41620469

PMCID

PMC12963404

PubMedCentral® Posted Date

2-1-2026

PubMedCentral® Full Text Version

Post-print

Abstract

The intrinsically photosensitive retinal ganglion cells (ipRGC) are the conduit between the retina and brain regions responsible for non-image-forming and image-forming vision. In mice, six ipRGC subtypes have been discovered based on morphological characteristics, functions, and molecular profiles. All ipRGCs arise from Tbr2-expressing RGCs during developmental stages and subsequently diverge and differentiate into the six mature, distinct subtypes in adult retinas. However, the cellular and molecular mechanisms controlling the formation and maturation of the six ipRGC subtypes remain elusive. Here, we demonstrate that two Tbr2-dependent transcription factors, Iroquois‑related homeobox 1 (Irx1) and T-box containing factor 20 (Tbx20), are key downstream transcription factors guiding lineage segregations of Tbr2-expressing RGC into distinct adult ipRGC subtypes. Both factors also control Opn4 expression. Irx1 is expressed in the M3, M4, and M5 subtypes, while Tbx20 is predominantly expressed in M1, M2, M6, and subgroups of M3 and M5. When Irx1 is ablated during retinal development, Opn4 expression is significantly reduced in the M3, M4, and M5 ipRGC groups; however, the formation of Irx1-expressing ipRGCs is not affected. In contrast, when Tbx20 is deleted, a significant number of Tbx20-expressing cells fail to develop while Opn4 expression is down-regulated. These findings reveal two parallel transcription cascades downstream of Tbr2 for controlling ipRGC subtype formation, fate divergence, and maintenance in the adult retina.

Keywords

Animals, T-Box Domain Proteins, Retinal Ganglion Cells, Mice, Homeodomain Proteins, Transcription Factors, Gene Expression Regulation, Developmental, Retina, Mice, Inbred C57BL, Developmental neurogenesis, Cell fate and cell lineage

Published Open-Access

yes

Recommended Citation

Kiyama, Takae; Chen, Ching-Kang; Altay, Halit Y; et al., "Tbr2-Dependent Parallel Pathways Regulate the Development of Distinct ipRGC Subtypes" (2026). Faculty, Staff and Student Publications. 6241.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/6241