Faculty, Staff and Student Publications
Publication Date
1-1-2024
Journal
Proceedings of SPIE - The International Society for Optical Engineering
DOI
10.1117/12.3007718
PMID
38827822
PMCID
PMC11141327
PubMedCentral® Posted Date
3-12-2025
PubMedCentral® Full Text Version
Author MSS
Abstract
Hyperspectral imaging (HSI) is a label-free imaging modality that is emerging for non-invasive detection of various diseases including cancers. HSI provides high-resolution spatial images where each pixel has a spectral curve with numerous wavelength bands from the visible to infrared ranges. The rich spatial and spectral information can be used to discriminate various types of tissues and pathophysiological conditions. However, it can be difficult to explain spectral data with respect to the underline cellular and molecular mechanism. In this study, we developed an approach that registers hyperspectral images and mass spectrometry (MS) data where MS provides tissue molecular profiles. Human prostate tissues that were obtained after prostatectomy were used in the experiments. The whole prostate was first sliced every six mm. A customized hyperspectral surgical microscope was used to acquire HSI data from the sliced tissue. For MS data analysis, the sliced tissue of the prostate was divided into 51 small regions and then processed separately for each region. The immediately adjacent tissue was sliced and processed histologically for H&E staining. The MS molecular profiles were correlated with the hyperspectral images in this study.
Keywords
hyperspectral imaging, mass spectrometry, registration, prostate cancer
Published Open-Access
yes
Recommended Citation
Angie T Fei, Douglas W Strand, and Jing Wang, "Registration of Hyperspectral Images and Mass Spectrometry Data for the Correlation of Tissue Optical Spectra and Molecular Profiles" (2024). Faculty, Staff and Student Publications. 6650.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/6650
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