Faculty, Staff and Student Publications
Publication Date
1-17-2024
Journal
Journal of Clinical Microbiology
DOI
10.1128/jcm.01096-23
PMID
38054730
PMCID
PMC10793330
PubMedCentral® Posted Date
December 2023
PubMedCentral® Full Text Version
Post-print
Abstract
Rapid diagnostic tests (RDTs) for bloodstream infections have the potential to reduce time to appropriate antimicrobial therapy and improve patient outcomes. Previously, an in-house, lipid-based, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) method, Fast Lipid Analysis Technique (FLAT MS), has shown promise as a rapid pathogen identification method. In this study, FLAT MS for direct from blood culture identification was evaluated and compared to FDA-cleared identification methods using the Benefit-risk Evaluation Framework (BED-FRAME) analysis. FLAT MS was evaluated and compared to Bruker Sepsityper and bioMérieux BioFire FilmArray BCID2 using results from a previous study. For this study, 301 positive blood cultures were collected from the University of Maryland Medical Center. The RDTs were compared by their sensitivities, time-to-results, hands-on time, and BED-FRAME analysis. The overall sensitivity of all platforms compared to culture results from monomicrobial-positive blood cultures was 88.3%. However, the three RDTs differed in their accuracy for identifying Gram-positive bacteria, Gram-negative bacteria, and yeast. Time-to-results for FLAT MS, Sepsityper, and BioFire BCID2 were all approximately one hour. Hands-on times for FLAT MS, Sepsityper, and BioFire BCID2 were 10 (±1.3), 40 (±2.8), and 5 (±0.25) minutes, respectively. BED-FRAME demonstrated that each RDT had utility at different pathogen prevalence and relative importance. BED-FRAME is a useful tool that can used to determine which RDT is best for a healthcare center.
Keywords
Humans, Bacteremia, Rapid Diagnostic Tests, Bacteriological Techniques, Sepsis, Blood Culture, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Lipids
Published Open-Access
yes
Recommended Citation
Smith, Richard D; Zhan, Min; Zhang, Shanshan; et al., "Comparison of Three Rapid Diagnostic Tests for Bloodstream Infections Using Benefit-Risk Evaluation Framework (BED-FRAME)" (2024). Faculty, Staff and Student Publications. 692.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/692
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Bioinformatics Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Biomedical Informatics Commons, Hematology Commons, Hemic and Lymphatic Diseases Commons, Medical Microbiology Commons, Oncology Commons