VHL Governs m6A Modification and PIK3R3 mRNA Stability in Clear Cell Renal Cell Carcinomas

Authors

Hyemin Lee
Li Zhuang
Boyi Gan

Publication Date

4-15-2024

Journal

The Journal of Clinical Investigation

Abstract

N6-Methyladenosine (m6A), a prevalent posttranscriptional modification, plays an important role in cancer progression. Clear cell renal cell carcinoma (ccRCC) is chiefly associated with the loss of the von Hippel-Lindau (VHL) gene, encoding a component of the E3 ubiquitin ligase complex. In this issue of the JCI, Zhang and colleagues unveiled a function of VHL beyond its canonical role as an E3 ubiquitin ligase in regulating hypoxia-inducible factors (HIFs). It also governed m6A modification by orchestrating the assembly of m6A writer proteins METTL3 and METTL14, thereby stabilizing PIK3R3 mRNA. Mechanistically, PIK3R3 contributed to p85 ubiquitination, which restrained PI3K/AKT signaling and consequently impeded ccRCC growth in cell and mouse models. This discovery provides potential treatment targets in VHL-deficient ccRCCs.

Keywords

Animals, Mice, Adenine, Carcinoma, Renal Cell, Kidney Neoplasms, Phosphatidylinositol 3-Kinases, RNA Stability, Ubiquitin-Protein Ligases, Humans

Comments

PMID: 38618953