CD45 and CD148 Are Critically Involved in Neutrophil Recruitment and Function During Inflammatory Arthritis in Mice

Authors

• Jan-Niklas Heming
• Andreas Margraf
• Karolina Najder
• Giulia Germena
• Mathis Richter
• Anika Cappenberg
• Katharina Henke
• Bernadette Bardel
• Lena Schemmelmann
• Marina Oguama
• Pia Lindental
• Wida Amini
• Jacqueline Sobocik
• Georg Schett
• Gerhard Krönke
• Helena Block
• Jan Rossaint
• Oliver Soehnlein
• Alexander Zarbock

Language

English

Publication Date

7-29-2025

Journal

Cells

DOI

10.3390/cells14151169

Abstract

Neutrophils play a key role in autoimmune diseases like rheumatoid arthritis, contributing to tissue damage through rapid recruitment and activation. In this study, we investigated the regulatory properties of two receptor-like tyrosine phosphatases (RPTPs), CD45 and CD148, in inflammatory arthritis. Using an in vivo mouse model of K/BxN serum transfer-induced arthritis, we found that CD45 and CD148 feature distinct regulatory properties during inflammatory arthritis. CD45 is required for neutrophil infiltration, cytokine release, and reactive oxygen species production, whereas CD148 deficiency leads to a delayed onset of arthritis but unaltered overall neutrophil infiltration and reduced ROS production. Furthermore, we could demonstrate that activation of Src family kinases in neutrophils is differentially regulated by CD45 and CD148 in a stimulus-dependent manner. Summarizing, our results suggest that CD45 is positively involved, while CD148 is positively and negatively involved in neutrophil recruitment and function during inflammatory arthritis.

Keywords

Animals, Mice, Leukocyte Common Antigens, Neutrophil Infiltration, Neutrophils, Mice, Inbred C57BL, Reactive Oxygen Species, src-Family Kinases, Arthritis, Experimental, Inflammation, Cytokines, arthritis, neutrophils, neutrophil recruitment, integrins, Src family kinases

Published Open-Access

yes

Recommended Citation

Heming, Jan-Niklas; Margraf, Andreas; Najder, Karolina; et al., "CD45 and CD148 Are Critically Involved in Neutrophil Recruitment and Function During Inflammatory Arthritis in Mice" (2025). Faculty, Staff and Student Publications. 3404.
https://digitalcommons.library.tmc.edu/uthmed_docs/3404