Delineating COVID-19 Immunological Features Using Single-Cell RNA Sequencing

Authors

Wendao Liu
Johnathan Jia
Yulin Dai
Wenhao Chen
Guangsheng Pei
Qiheng Yan
Zhongming Zhao

Publication Date

9-13-2022

Journal

Innovation

Abstract

Understanding the molecular mechanisms of coronavirus disease 2019 (COVID-19) pathogenesis and immune response is vital for developing therapies. Single-cell RNA sequencing has been applied to delineate the cellular heterogeneity of the host response toward COVID-19 in multiple tissues and organs. Here, we review the applications and findings from over 80 original COVID-19 single-cell RNA sequencing studies as well as many secondary analysis studies. We describe that single-cell RNA sequencing reveals multiple features of COVID-19 patients with different severity, including cell populations with proportional alteration, COVID-19-induced genes and pathways, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in single cells, and adaptation of immune repertoire. We also collect published single-cell RNA sequencing datasets from original studies. Finally, we discuss the limitations in current studies and perspectives for future advance.

DOI

10.1016/j.xinn.2022.100289

PMID

35879967

PMCID

PMC9299978

PubMedCentral® Posted Date

7-21-2022

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes