Student and Faculty Publications
Publication Date
10-13-2023
Journal
Frontiers in Immunology
Abstract
INTRODUCTION: The tumor microbiome (TM) has been linked to pancreatic cancer prognosis. Specific microbes can confer tumor resistance to therapies. Early knowledge of the TM at time of diagnosis would be clinically relevant for precision therapy based on microbial composition. However, it is difficult to define the TM prior to surgical resection.
METHODS: In this pilot feasibility study, patients underwent Endoscopic Ultrasound-Fine Needle Aspiration (EUS-FNA) biopsy of pancreatic adenocarcinoma. These samples were analyzed using 16S rRNA and internal transcribed spacer (ITS) sequencing for characterization of the tumor bacteria and fungi.
RESULT: After in silico decontamination and comparison to non-matched tumor, we were able to characterize the TM in biopsies, which was comparable to the TM from surgical specimens.
DISCUSSION: EUS-FNA biopsy may represent a feasible modality to characterize the pancreatic TM prior to surgical resection with proper decontamination strategies and improvements in matched controls.
Keywords
Humans, Pancreatic Neoplasms, Adenocarcinoma, RNA, Ribosomal, 16S, Endoscopic Ultrasound-Guided Fine Needle Aspiration, Bacteria, tumor microbes, pancreatic cancer, endoscopic ultrasound, fine needle aspiration, microbiome, pancreatic adenocarcinoma
Included in
Bioinformatics Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Biomedical Informatics Commons, Oncology Commons
Comments
Supplementary Materials
PMID: 37901238