A Comparative Analysis of the Immunoglobulin Repertoire in Leukemia Cells and B Cells in Chinese Acute Myeloid Leukemia by High-Throughput Sequencing

Authors

Huige Yan
Lina Wu
Pingzhang Wang
Miaoran Xia
Zhan Shi
Xinmei Huang
Sha Yin
Qian Jiang
C Cameron Yin
Xiangyu Zhao
Xiaoyan Qiu

Publication Date

8-13-2024

Journal

Biology

DOI

10.3390/biology13080613

PMID

39194551

PMCID

PMC11351582

PubMedCentral® Posted Date

August 2024

PubMedCentral® Full Text Version

Post-print

Abstract

It is common knowledge that immunoglobulin (Ig) is produced by B lymphocytes and mainly functions as an antibody. However, it has been shown recently that myeloblasts from acute myeloid leukemia (AML) could also express Ig and that AML-Ig played a role in leukemogenesis and AML progression. The difference between Ig from myeloblasts and B cells has not been explored. Studying the characteristics of the Ig repertoire in myeloblasts and B cells will be helpful to understand the function and significance of AML-Ig. We performed 5' RACE-related PCR coupled with PacBio sequencing to analyze the Ig repertoire in myeloblasts and B cells from Chinese AML patients. Myeloblasts expressed all five classes of IgH, especially Igγ, with a high expression frequency. Compared with B-Ig in the same patient, AML-Ig showed different biased V(D)J usages and mutation patterns. In addition, the CDR3 length distribution of AML-Ig was significantly different from those of B-Ig. More importantly, mutations of AML-IgH, especially Igμ, Igα, and Igδ, were different from that of B-IgH in each AML patient, and the mutations frequently occurred at the sites of post-translational modification. AML-Ig has distinct characteristics of variable regions and mutations, which may have implications for disease monitoring and personalized therapy.

Keywords

acute myeloid leukemia, myeloblast, immunoglobulin, V(D)J rearrangement patterns, somatic hypermutation, B cells

Published Open-Access

yes

Recommended Citation

Yan, Huige; Wu, Lina; Wang, Pingzhang; et al., "A Comparative Analysis of the Immunoglobulin Repertoire in Leukemia Cells and B Cells in Chinese Acute Myeloid Leukemia by High-Throughput Sequencing" (2024). Faculty, Staff and Student Publications. 2432.
https://digitalcommons.library.tmc.edu/uthgsbs_docs/2432