Faculty, Staff and Student Publications

Language

English

Publication Date

10-17-2025

Journal

iScience

DOI

10.1016/j.isci.2025.113569

PMID

41079605

PMCID

PMC12510045

PubMedCentral® Posted Date

9-12-2025

PubMedCentral® Full Text Version

Post-print

Abstract

Trauma remains a leading cause of morbidity and mortality in part due to complex pathophysiological responses. Yet our abilities to predict these changes are limited. Plasma miRNAs have been proposed as DAMPs that drive immune response and organ injury. Here, we test a panel of plasma miRNAs-selected based on next-generation sequencing and nucleotide motifs identified via a machine-learning algorithm-for their abilities to predict subclinical pathophysiological injuries. We find marked and severity-dependent increases in the miRNA biomarkers following trauma, which are closely associated with various injury markers. AUROC indicates that these biomarkers possess strong diagnostic and predictive abilities in overall trauma severity, organ injury, coagulation, endothelial activation, and inflammation. In a combined cohort of trauma and sepsis, miR-224-5p and miR-145-5p emerge as particularly effective in differentiating the two critical illnesses. These observations offer insights into potential values of the plasma miRNAs in the prediction of critical pathophysiological injury in trauma.

Keywords

Diagnostics, Health sciences, Internal medicine, Medicine, Traumatology

Published Open-Access

yes

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